There will be a detour on the section of the Yarra Trail along the Melbourne Convention and Exhibition Centre (MCEC) from Friday 6 October to Sunday 8 October.
Bike riders will be able to continue along the Yarra as the boardwalk along the river's edge in Polly Woodside Park will be open to people on bikes.
Large crowds are expected at the MCEC for the PAX gaming festival and queue control measures will see closure of the path adjacent to the centre.
Some riders may recall this detour also being used on Good Friday earlier this year.