A popular section of the Main Yarra Trail between Gipps Street and the Clarke Street connection in Abbotsford will be temporarily closed for five days next week.
Parks Victoria is undertaking soil testing at a number of locations along the stretch of trail, close to the path.
Riders will be directed on an alternative route via Gipps Street, Nicholson Street, Yarra Street and Clarke Street.
Access to the Collins Bridge will be maintained.
The trail will be closed each day between 8:00am and 4:00pm, Monday to Friday.