Level Crossing works will close bike lanes in Dandenong for three weeks from Friday.
The bike lanes between South Gippsland Highway and Princes Highway will be closed.
Riders can detour via Dandenong Bypass and Frankston-Dandenong Road.
The access changes will be in places from 9pm Friday 16 July to 5am Friday 6 August.
Pedestrian access on the south side of the Princes Highway and South Gippsland Highway junction will be also be closed.
Pedestrians can detour via Lonsdale Street, Frankston-Dandenong Road and Dandenong Bypass.