Buses replace trains on the Frankston Line between Caulfield and Moorabbin from 8:30 pm Wednesday 24 August until 4 am Saturday 27 August, while Level Crossing Removal works take place.
Riders who usually take their bikes on the train will be unable to take them on the substitute buses.
Major construction is taking place as part of Victoria's Big Build, with the removal of two level crossings at Glen Huntly Road and Neerim Road taking place.